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Amplifx primer list format2/28/2023 ![]() Windows users will also have to change some default settings of the Virtual Machines. To use the 2nd variant, download docker image of NGS-PrimerPlex in the following way:ĭocker pull aakechin/ngs-primerplex:native To use 1st variant, download docker image of NGS-PrimerPlex with the following command:ĭocker pull aakechin/ngs-primerplex:latest In this case you will have to download about only 0.5 Gb, but you also have to download reference genome files manually (see section "Reference genome for other organisms than human"). The 2nd is ideal for use with other reference genomes, including other organisms. ![]() The 1st variant is idead for use with hg19 genome, but you will have to download about 8 Gb of data. Now users have two options of NGS-PrimerPlex use in docker: (1) with already uploaded human reference genome hg19 version, (2) without any reference genomes. If you have "VD-x, VD-t error", you need to turn on virtualization in BIOS CPU section. In this way you only need to install Docker (for windows 7 users this install steps should be performed). NGS-PrimerPlex can be run as a Docker image. convertToDraftFile.py - script that converts the main NGS-PrimerPlex output file into the draft-primers file.addSeqToPrimers.py - script that adds adapter sequences for all of the designed primers.NGS_primerplex.py - script that designs primers for the user defined genome coordinates.getGeneRegions.py - script that converts list of genes and their parts into genome coordinates for which the next script designs primers.NGS-PrimerPlex is a high-throughput tool for mupltiplex primer design
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